Serine carboxypeptidases from the carnivorous plant Nepenthes mirabilis: Partial characterization and heterologous expression.

International journal of biological macromolecules 2022 Feb 15

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This study aimed to partially characterize the three main serine carboxypeptidases (SCP3, SCP20, and SCP47) from Nepenthes mirabilis. Furthermore, one peptidase (SCP3) was chosen for further heterologous expression in Escherichia coli Shuffle®T7. SCP3 also was characterized in terms of its allergenic potential using bioinformatics tools. SCP3, SCP20, and SCP47 showed very similar 3D structures and mechanistic features to other plant serine peptidases belonging to clan SC and family S10. Although SCP3 was obtained in its soluble form, using 1% ethanol during induction with 0.5 mM IPTG at 16 °C for 18 h, it did not show proteolytic activity by zymography or in vitro analysis. SCP3 presented a few allergenic peptides and several cleavage sites for digestive enzymes. This work describes additional features of these enzymes, opening new perspectives for further studies for characterization and analysis of heterologous expression, as well as their potential biotechnological applications. Copyright © 2021 Elsevier B.V. All rights reserved.

Citation

Camila T M N Porfírio, Pedro F N Souza, Márcio V Ramos, Francisco A P Campos, Samuel F Freitas, João P B Oliveira, Gilvan P Furtado, José S S Barbosa, Thalia L Frota, Celso S Nagano, Rodolpho G G Silva, Ghulam Hussain, Cleverson D T Freitas. Serine carboxypeptidases from the carnivorous plant Nepenthes mirabilis: Partial characterization and heterologous expression. International journal of biological macromolecules. 2022 Feb 15;198:77-86