Structural characterization and hepatoprotective effects of polysaccharides from Anoectochilus zhejiangensis.

Two new polysaccharides, AZP-1a and AZP-1d, with molecular weights of 3.41 × 104 and 4568 Da, respectively, were extracted from Anoectochilus zhejiangensis and purified by column chromatography. Their structural characteristics were systematically explored and results indicated AZP-1a and AZP-1d shared a similar backbone consisted of→4)-Galp-(1→, →4)-Glcp-(1→, and →4,6)-Glcp-(1→, with a different terminal residue of Manp-(1 → and Glcp-(1→, respectively. In vivo experiments showed that the crude polysaccharide of A. zhejiangensis (AZP) exhibited significant hepatoprotective effects, decreasing the serum levels of ALT, AST and LDH in CCl4-treated mice, reducing MDA content, promoting SOD and CAT activities, and increasing GSH level in liver. Further in vitro investigation exhibited that AZP, AZP-1a and AZP-1d effectively protected liver cells against CCl4-stimulated oxidative damage, while AZP-1a and AZP-1d functioned mainly through the activation of Nrf2 signaling pathway. Our results suggest that A. zhejiangensis polysaccharides can be applied as a potential resource for the development of hepatoprotective drugs. Copyright © 2021 Elsevier B.V. All rights reserved.

Citation

Yanbin Wu, Chang Liu, Yingqian Jiang, Bingke Bai, Xuhui He, Hongrui Wang, Jinzhong Wu, Chengjian Zheng. Structural characterization and hepatoprotective effects of polysaccharides from Anoectochilus zhejiangensis. International journal of biological macromolecules. 2022 Feb 15;198:111-118

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PMID: 34968535

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