Preparation and Screening of Cell-Free Extract from Nongrowing Escherichia coli A19 Cells.

Florian Hiering, Jurek Failmezger, Martin Siemann-Herzberg

Methods in molecular biology (Clifton, N.J.) 2022

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Cell-free extracts have been researched and continuously streamlined for around 50 years. It is believed that these extracts work best when routinely obtained from exponentially growing cells to capture the most active translation system. Here we report on an active cell-free extract derived from E. coli A19 that was harvested under nongrowing, stressed conditions. Although this process is based on the conventional routine process for the production of S30-extracts, our process is less labor intensive and reduces variability between extracts. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Florian Hiering, Jurek Failmezger, Martin Siemann-Herzberg. Preparation and Screening of Cell-Free Extract from Nongrowing Escherichia coli A19 Cells. Methods in molecular biology (Clifton, N.J.). 2022;2433:65-73