Giant plasma membrane vesicles to study plasma membrane structure and dynamics.

Erdinc Sezgin

Biochimica et biophysica acta. Biomembranes 2022 Apr 01

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The plasma membrane (PM) is a highly heterogenous structure intertwined with the cortical actin cytoskeleton and extracellular matrix. This complex architecture makes it difficult to study the processes taking place at the PM. Model membrane systems that are simple mimics of the PM overcome this bottleneck and allow us to study the biophysical principles underlying the processes at the PM. Among them, cell-derived giant plasma membrane vesicles (GPMVs) are considered the most physiologically relevant system, retaining the compositional complexity of the PM to a large extent. GPMVs have become a key tool in membrane research in the last few years. In this review, I will provide a brief overview of this system, summarize recent applications and discuss the limitations. Copyright © 2022 The Author. Published by Elsevier B.V. All rights reserved.

Citation

Erdinc Sezgin. Giant plasma membrane vesicles to study plasma membrane structure and dynamics. Biochimica et biophysica acta. Biomembranes. 2022 Apr 01;1864(4):183857