Expression, purification and microscopic characterization of transmembrane BAX Inhibitor-1 motif containing protein 5.

Yue Lu, Xuhang Lu, Yingjian Xu, Yue Ren, Yuequan Shen, Xue Yang

Protein expression and purification 2022 May

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Transmembrane bax inhibitor-1 motif containing protein 5 (TMBIM5) is located on the inner membrane of mitochondria and is widely expressed in tissues but less frequently in the intestine and thymus. TMBIM5 affects mitochondrial cristae organization and is associated with Parkinson's disease. Here, we present the first report about expression, purification and the 2D classification projections derived from negatively stained electron micrographs of recombinant H. sapiens TMBIM5 (hTMBIM5). The described methods and results will support further structural and functional study of hTMBIM5. Copyright © 2022 Elsevier Inc. All rights reserved.

Citation

Yue Lu, Xuhang Lu, Yingjian Xu, Yue Ren, Yuequan Shen, Xue Yang. Expression, purification and microscopic characterization of transmembrane BAX Inhibitor-1 motif containing protein 5. Protein expression and purification. 2022 May;193:106045