Long noncoding RNAs (lncRNAs) are transcripts over 200 base pairs in length without discernible protein coding potential. Long intergenic noncoding RNAs (lincRNAs) constitute a subset of lncRNAs, which do not overlap protein coding genes. Here we describe a detailed pipeline for lincRNA discovery from publicly available non-stranded RNA-Seq datasets. The pipeline presented can be applied to any plant species for which RNA-Seq data and a reference genome sequence are available. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
A A Golicz. Long Intergenic Noncoding RNA (lincRNA) Discovery from Non-Strand-Specific RNA-Seq Data. Methods in molecular biology (Clifton, N.J.). 2022;2443:465-482
PMID: 35037221
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