Direct excitation of tryptophan phosphorescence. A new method for triplet states investigation.

We studied room temperature phosphorescence of tryptophan (TRP) embedded in poly (vinyl alcohol) films. With UV (285 nm) excitation, the phosphorescence spectrum of tryptophan appears at about 460 nm. We also observed the TRP phosphorescence with blue light excitation at 410 nm, well outside of the S0→S1absorption. This excitation reaches the triplet state of tryptophan directly without the involvement of the singlet excited state. The phosphorescence lifetime of tryptophan is in the sub-millisecond range. The long-wavelength direct excitation to the triplet state results in high phosphorescence anisotropy which can be useful in macromolecule dynamics study via time-resolved phosphorescence. © 2022 IOP Publishing Ltd.

Citation

Jose Chavez, Luca Ceresa, John M Reeks, Yuri M Strzhemechny, Joseph Kimball, Emma Kitchner, Zygmunt Gryczynski, Ignacy Gryczynski. Direct excitation of tryptophan phosphorescence. A new method for triplet states investigation. Methods and applications in fluorescence. 2022 Feb 02;10(2)

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PMID: 35042210

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