Bidirectional Transport of IgE by CD23 in the Inner Ear of Patients with Meniere's Disease.

Na Zhang, Yafeng Lyu, Jia Guo, Jiahui Liu, Yongdong Song, Zhaomin Fan, Xiaofei Li, Na Li, Daogong Zhang, Haibo Wang

Journal of immunology (Baltimore, Md. : 1950) 2022 Feb 15

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Meniere's disease (MD) is a disorder of the inner ear characterized by episodes of spontaneous vertigo, fluctuating hearing loss, and tinnitus. Recent studies have demonstrated that IgE may play a role in the pathogenesis of MD. Patients with MD (n = 103), acoustic neuroma (n = 5), and healthy subjects (n = 72) were recruited into the study. Serum from the participants was analyzed for IgE and type 2-related cytokines. IgE and CD23 expression levels in vestibular end organs of patients, C57BL/6 mice, or mouse HEI-OC1 cells were analyzed. Finally, the role of CD23 in IgE transcytosis was assessed using HEI-OC1 cells. Serum IgE was elevated in patients with MD and positively correlated with clinical symptoms. IL-4, IL-5, IL-10, IL-13, and CD23 levels were increased in patients with MD compared with the control group. In the transcytosis assay, mouse IgE was found to be bidirectionally transported across the HEI-OC1 cell monolayer. Additionally, CD23 downregulation using a small interfering RNA approach significantly reduced the efficiency of IgE transcytosis, suggesting that IgE is transported by CD23. Furthermore, exposure to IL-4 increased CD23 expression and enhanced IgE transcytosis in the HEI-OC1 cells and primary vestibular end organs. Our study indicated that IgE may play a role in the pathophysiology of MD. In addition, CD23-mediated IgE transcytosis in the hair cells may play a critical role in initiating inflammation in the inner ear. Thus, reducing the level of IgE may be a potentially effective approach for MD treatment. Copyright © 2022 by The American Association of Immunologists, Inc.

Citation

Na Zhang, Yafeng Lyu, Jia Guo, Jiahui Liu, Yongdong Song, Zhaomin Fan, Xiaofei Li, Na Li, Daogong Zhang, Haibo Wang. Bidirectional Transport of IgE by CD23 in the Inner Ear of Patients with Meniere's Disease. Journal of immunology (Baltimore, Md. : 1950). 2022 Feb 15;208(4):827-838