Immunization of mice with the self-peptide ACBP coupled to keyhole limpet hemocyanin.

Léa Montégut, Hui Chen, José Manuel Bravo-San Pedro, Omar Motiño, Isabelle Martins, Guido Kroemer

STAR protocols 2022 Mar 18

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Keyhole limpet hemocyanin (KLH) is a glycosylated multi-subunit metalloprotein that elicits a strong nonspecific immune activation, thus inducing both cellular and humoral immune responses. The exceptional immunogenicity of this protein can be leveraged to vaccinate mice against self-antigens that otherwise would not induce an autoimmune response. This protocol describes the covalent conjugation of KLH with acyl-coenzyme A-binding protein (ACBP), the autovaccination of mice with ACBP-KLH conjugate together with a potent adjuvant, and the detection of the produced anti-ACBP autoantibodies. For complete details on the use and execution of this profile, please refer to Bravo-San Pedro et al. (2019c). © 2021 The Author(s).

Citation

Léa Montégut, Hui Chen, José Manuel Bravo-San Pedro, Omar Motiño, Isabelle Martins, Guido Kroemer. Immunization of mice with the self-peptide ACBP coupled to keyhole limpet hemocyanin. STAR protocols. 2022 Mar 18;3(1):101095