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    Red-to-NIR absorption and emission wavelengths are key requirements for intravital bioimaging. One of the way to reach such excitation wavelengths is to use two-photon excitation. Unfortunately, there is still a lack of two-photon excitable fluorophores that are both efficient and biocompatible. Thus, we design a series of biocompatible quadrupolar dyes in order to study their ability to be used for live-cell imaging, and in particular for two-photon microscopy. Hence, we report the synthesis of 5 probes based on different donor cores (phenoxazine, acridane, phenazasiline and phenothiazine) and the study of their linear and non-linear photophysical properties. TD-DFT calculations were performed and were able to highlight the structure-property relationship of this series. All these studies highlight the great potential of three of these biocompatible dyes for two-photon microscopy, as they both exhibit high two-photon cross-sections (up to 3650 GM) and emit orange to red light. This potential was confirmed through live-cell two-photon microscopy experiments, leading to images with very high brightness and contrast. © 2022 Wiley-VCH GmbH.


    Marie Auvray, Frédéric Bolze, Delphine Naud-Martin, Matthieu Poulain, Margaux Bossuat, Gilles Clavier, Florence Mahuteau-Betzer. On the Road Toward More Efficient Biocompatible Two-Photon Excitable Fluorophores. Chemistry (Weinheim an der Bergstrasse, Germany). 2022 Mar 07;28(14):e202104378

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    PMID: 35060213

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