Responses of lipid metabolism and lipidomics in the hepatopancreas of Pacific white shrimp Litopenaeus vannamei to microcystin-LR exposure.

Yafei Duan, Shimin Zeng, Zijun Lu, Xueming Dan, Zequan Mo, Yifu Xing, Jiasong Zhang, Yanwei Li

The Science of the total environment 2022 May 10

filter terms:

Microcystin-LR (MC-LR) is a toxic substance that threatens the health of aquatic animals. Hepatopancreas is the target organ of MC-LR toxicity. In this study, we investigated the effects of MC-LR on hepatopancreas lipid metabolism and lipidomic responses in Litopenaeus vannamei. After MC-LR exposure for 72 h, the hepatopancreas showed obvious tissue damage, and the activities of several lipase isoenzymes were decreased. Furthermore, the relative gene expression levels of lipolysis (CPT1, AMPKα), lipogenesis (SREBP, FAS, ACC, 6PGD), and long-chain fatty acid β-oxidation (ACDL, ACDVL, ACBP) were increased. MC-LR exposure also affected lipidomics homeostasis. Specifically, the levels of glycerophospholipids (phosphatidylcholine, phosphatidic acid, lyso-phosphatidylcholine, lyso-phosphatidylethanolamine, lyso-phosphatidylglycerol), sphingolipids (sphingomyelin and ceramides) and cholesteryl ester were increased, and those of phosphatidylinositol and triglyceride were decreased. The significantly altered lipid molecules were mainly associated with the pathways of lipid and fatty acid metabolism and autophagy. These results reveal that MC-LR exposure influences lipid metabolism and lipidomic homeostasis in the shrimp hepatopancreas. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Yafei Duan, Shimin Zeng, Zijun Lu, Xueming Dan, Zequan Mo, Yifu Xing, Jiasong Zhang, Yanwei Li. Responses of lipid metabolism and lipidomics in the hepatopancreas of Pacific white shrimp Litopenaeus vannamei to microcystin-LR exposure. The Science of the total environment. 2022 May 10;820:153245