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    This study evaluated the color change, enamel surface roughness and microhardness after different tooth bleaching protocols, using hydrogen peroxide (HP) and/or violet LED. Forty bovine specimens (7 × 7 × 2 mm) were randomly distributed into 4 groups: 35% HP, 6% HP, 6% HP + violet LED and violet LED alone. First, the specimens were stained with black tea and then submitted to two bleaching sessions of 30 min with an interval of 7 days. Color change (∆L*, ∆a*, ∆b* and ∆E00) after 24 h of each session and 1 week after the last session was evaluated. Enamel roughness and microhardness were evaluated immediately before the sessions, 24 h and 1 week after the last session. Data were evaluated by ANOVA for repeated measures and Bonferroni post-test or Kruskall-Wallis and Dunn tests (α = 0.05). Representative specimens from each group were analyzed by scanning electron microscopy. 6% HP + violet LED and 35% HP showed the highest color change, while violet LED alone had the lowest results. Enamel roughness analyses showed that 6% HP + violet LED and 35% HP showed changes after two bleaching sessions. No differences were observed regarding enamel microhardness. The use of 6% HP + violet LED showed enhanced bleaching efficacy compared to 35% HP. However, violet LED used alone exhibited the lowest color change. 6% HP + violet LED and 35% HP promoted changes on enamel roughness, while no microhardness changes was observed for any group. Copyright © 2022 Elsevier B.V. All rights reserved.


    Joatan Lucas de Sousa Gomes Costa, João Felipe Besegato, Joissi Ferrari Zaniboni, Lucas David Galvani, Milton Carlos Kuga. Effects of tooth bleaching protocols with violet LED and hydrogen peroxide on enamel properties. Photodiagnosis and photodynamic therapy. 2022 Jun;38:102733

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    PMID: 35065278

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