Bioproduction of Natural Phenethyl Acetate, Phenylacetic Acid, Ethyl Phenylacetate, and Phenethyl Phenylacetate from Renewable Feedstock.

Balaji Sundara Sekar, Xirui Li, Zhi Li

ChemSusChem 2022 May 06

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Natural phenethyl acetate (PEA), phenylacetic acid (PAA), ethyl phenylacetate (Et-PA), and phenethyl phenylacetate (PE-PA) are highly desirable aroma chemicals, but with limited availability and high price. Here, green, sustainable, and efficient bioproduction of these chemicals as natural products from renewable feedstocks was developed. PEA and PAA were synthesized from l-phenylalanine (l-Phe) via novel six- and five-enzyme cascades, respectively. Whole-cell-based cascade biotransformation of 100 mm l-Phe in a two-phase system (aqueous/organic: 1 : 0.5 v/v) containing ethyl oleate or biodiesel as green solvent gave 13.6 g L-1 PEA (83.1 % conv.) and 11.6 g L-1 PAA (87.1 % conv.), respectively. Coupled fermentation and biotransformation approach produced 10.4 g L-1 PEA and 9.2 g L-1 PAA from glucose or glycerol, respectively. The biosynthesized PAA was converted to natural Et-PA and PE-PA by esterification using lipases with ethanol or 2-phenylethanol derived from sugar, affording 2.7 g L-1 Et-PA (83.1 % conv.) and 4.6 g L-1 PE-PA (96.3 % conv.), respectively. © 2022 Wiley-VCH GmbH.

Citation

Balaji Sundara Sekar, Xirui Li, Zhi Li. Bioproduction of Natural Phenethyl Acetate, Phenylacetic Acid, Ethyl Phenylacetate, and Phenethyl Phenylacetate from Renewable Feedstock. ChemSusChem. 2022 May 06;15(9):e202102645