Determining the metabolic impact of postbiotics in mice.

Fernando F Anhê, Arshpreet Bhatwa, Jonathan D Schertzer

STAR protocols 2022 Mar 18

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Postbiotics cooperate to influence immune and metabolic outcomes in the host. Here we describe a protocol for in vivo assessment of blood glucose control following acute administration of lipopolysaccharide (LPS) and peptidoglycan (PGN) in mice. This protocol can be adapted for testing a broad range of microbial molecules and ligands for host immune receptors. Experience with mouse handling is required. For complete details on the use and execution of this protocol, please refer to Anhê et al. (2021) and Cavallari et al. (2017). © 2021 The Author(s).

Citation

Fernando F Anhê, Arshpreet Bhatwa, Jonathan D Schertzer. Determining the metabolic impact of postbiotics in mice. STAR protocols. 2022 Mar 18;3(1):101098