Jennifer Londregan, Jeffrey Maslanka, Naomi Goldman, John Somerville, James E Riggs
Immunobiology 2022 MarAtypical cytokine production and immune cell subset ratios, particularly those that include high proportions of macrophages, characterize tumor microenvironments (TMEs). TMEs can be modeled by culturing peritoneal cavity (PerC) cells which have a high macrophage to lymphocyte ratio. With TCR or BCR ligation, PerC lymphocyte proliferation is tempered by macrophages. However, PHA (T cells) and anti-CD40 (B cells) are activators that induce proliferation. Herein, we report that ligating IgD, in contrast to IgM, triggers PerC B cell proliferation. IL-4 addition enhanced the IgD response for BALB/c PerC B cells but suppressed that of C57BL/6 mice. Intriguingly, concurrent ligation of IgD and CD3ε rescued a PerC T cell proliferative response. These results serve to expand the list of targets for promoting cellular and humoral immunity in conditions that model macrophage-rich TMEs. Copyright © 2022 Elsevier GmbH. All rights reserved.
Jennifer Londregan, Jeffrey Maslanka, Naomi Goldman, John Somerville, James E Riggs. IgD ligation allows peritoneal cavity B cell proliferation. Immunobiology. 2022 Mar;227(2):152181
PMID: 35077917
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