Xylazole inhibits NO-cGMP pathway in fetal rat nerve cells.

Xinyu Wang, Yue Wu, Lin Liu, Hui Bai, Zhiheng Zhang, Mingchao Zhao, Tianwen Ma, Xiaopeng Song, Lina Jia, Liangyu Lv, Yue Yu, Xinyu Xu, Hong Chen, Li Gao

Journal of veterinary science 2022 Jan

filter terms:

Xylazole (Xyl) is a veterinary anesthetic that is structurally and functionally similar to xylazine. However, the effects of Xyl in vitro remain unknown. This study aimed to investigate the anesthetic mechanism of Xyl using fetal rat nerve cells treated with Xyl. Fetal rat nerve cells cultured for seven days were treated with 10, 20, 30, and 40 μg/ mL Xyl for 0, 5, 10, 15, 20, 25, 30, 45, 60, 90, and 120 min. Variations of amino acid neurotransmitters (AANTs), Nitric oxide-Cyclic GMP (NO-cGMP) signaling pathway, and ATPase were evaluated. Xyl decreased the levels of cGMP and NO in nerve cells. Furthermore, Xyl affected the AANT content and Na+-K+-ATPase and Ca2+-Mg2+-ATPase activity in nerve cells. These findings suggested that Xyl inhibited the NO-cGMP signaling pathway in nerve cells in vitro. This study provided new evidence that the anesthetic and analgesic effects of Xyl are related to the inhibition of the NO-cGMP signaling pathway. © 2022 The Korean Society of Veterinary Science.

Citation

Xinyu Wang, Yue Wu, Lin Liu, Hui Bai, Zhiheng Zhang, Mingchao Zhao, Tianwen Ma, Xiaopeng Song, Lina Jia, Liangyu Lv, Yue Yu, Xinyu Xu, Hong Chen, Li Gao. Xylazole inhibits NO-cGMP pathway in fetal rat nerve cells. Journal of veterinary science. 2022 Jan;23(1):e16