Correlation Engine 2.0
Clear Search sequence regions

  • brain (5)
  • drosophila (1)
  • exons (2)
  • humans (1)
  • introns (1)
  • mice (3)
  • neurons (1)
  • rna (3)
  • rna precursors (3)
  • Sizes of these terms reflect their relevance to your search.

    Recursive splicing (RS) is a splicing mechanism to remove long introns from messenger RNA precursors of long genes. Compared to the hundreds of RS events identified in humans and drosophila, only ten RS events have been reported in mice. To further investigate RS in mice, we analyzed RS in the mouse brain, a tissue that is enriched in the expression of long genes. We found that nuclear total RNA sequencing is an efficient approach to investigate RS events. We analyzed 1.15 billion uniquely mapped reads from the nuclear total RNA sequencing data in the mouse cerebral cortex. Unexpectedly, we only identified 20 RS sites, suggesting that RS is a rare event in the mouse brain. We also identified that RS is constitutive between excitatory and inhibitory neurons and between sexes in the mouse cerebral cortex. In addition, we found that the primary sequence context is associated with RS splicing intermediates and distinguishes RS AGGT site from non-RS AGGT sites, indicating the importance of the primary sequence context in RS sites. Moreover, we discovered that cryptic exons may use an RS-like mechanism for splicing. Overall, we provide novel findings about RS in long genes in the mouse brain.


    Sohyun Moon, Ying-Tao Zhao. Recursive splicing is a rare event in the mouse brain. PloS one. 2022;17(1):e0263082

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 35089962

    View Full Text