Natalia Nakamura-Gouvea, Cicero Alves-Lima, Luiz Felipe Benites, Cintia Iha, Vinicius Maracaja-Coutinho, Victor Aliaga-Tobar, Marcella Araujo Amaral Carneiro, Nair S Yokoya, Eliane Marinho-Soriano, Marcia A S Graminha, Jonas Collén, Mariana C Oliveira, Joao C Setubal, Pio Colepicolo
Journal of phycology 2022 JunGracilariales is a clade of florideophycean red macroalgae known for being the main source of agar. We present a de novo genome assembly and annotation of Gracilaria domingensis, an agarophyte alga with flattened thallus widely distributed along Central and South American Atlantic intertidal zones. In addition to structural analysis, an organizational comparison was done with other Rhodophyta genomes. The nuclear genome has 78 Mbp, with 11,437 predicted coding genes, 4,075 of which did not have hits in sequence databases. We also predicted 1,567 noncoding RNAs, distributed in 14 classes. The plastid and mitochondrion genome structures were also obtained. Genes related to agar synthesis were identified. Genes for type II galactose sulfurylases could not be found. Genes related to ascorbate synthesis were found. These results suggest an intricate connection of cell wall polysaccharide synthesis and the redox systems through the use of L-galactose in Rhodophyta. The genome of G. domingensis should be valuable to phycological and aquacultural research, as it is the first tropical and Western Atlantic red macroalgal genome to be sequenced. © 2022 Phycological Society of America.
Natalia Nakamura-Gouvea, Cicero Alves-Lima, Luiz Felipe Benites, Cintia Iha, Vinicius Maracaja-Coutinho, Victor Aliaga-Tobar, Marcella Araujo Amaral Carneiro, Nair S Yokoya, Eliane Marinho-Soriano, Marcia A S Graminha, Jonas Collén, Mariana C Oliveira, Joao C Setubal, Pio Colepicolo. Insights into agar and secondary metabolite pathways from the genome of the red alga Gracilaria domingensis (Rhodophyta, Gracilariales). Journal of phycology. 2022 Jun;58(3):406-423
PMID: 35090189
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