Charge-Based Multiarm Avidin Nanoconstruct as a Platform Technology for Applications in Drug Delivery.

Tengfei He, Chenzhen Zhang, Ambika G Bajpayee

Methods in molecular biology (Clifton, N.J.) 2022

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Here we describe methods for synthesizing cationic multiarm Avidin (mAv) nanoconstruct that has a wide range of applications in drug delivery and imaging for a variety of negatively charged tissues. The multiarm structure provides multiple sites for covalent conjugation of drugs. We use avidin-biotin reaction that gives the flexibility for conjugating any desired biotinylated drug to mAv by simple mixing at room temperature. We also describe methods to control hydrolysis rates of ester linkers to enable sustained (and tunable) drug release rates in therapeutic doses. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Tengfei He, Chenzhen Zhang, Ambika G Bajpayee. Charge-Based Multiarm Avidin Nanoconstruct as a Platform Technology for Applications in Drug Delivery. Methods in molecular biology (Clifton, N.J.). 2022;2394:537-553