Methods for Forming Human Lymphatic Microvessels In Vitro and Assessing their Drainage Function.

Joe Tien, Usman Ghani

Methods in molecular biology (Clifton, N.J.) 2022

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This chapter describes methods to engineer human lymphatic microvessels in vitro and to assess their fluid and solute drainage capacities. The lymphatics are formed within micropatterned type I collagen gels that contain a blind-ended channel for the growth of lymphatic endothelial cells. Because the vessels have one blind end and one open end each, they mimic the terminal structure of the native lymphatic microvascular tree. The solute drainage rates that are measured from the engineered lymphatics in vitro can be directly compared with published results from intact vessels in vivo. Practical considerations to increase the accuracy of the drainage assays are discussed. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Joe Tien, Usman Ghani. Methods for Forming Human Lymphatic Microvessels In Vitro and Assessing their Drainage Function. Methods in molecular biology (Clifton, N.J.). 2022;2394:651-668