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    Purkinje neurons (PNs) are an important component of the motor learning and coordination circuit and are affected in spino-cerebellar ataxias. Maintaining healthy PNs in cerebellar slices and recording their Ca2+ transients can be challenging. Here, we describe a protocol for measuring Ca2+ transients in PNs from adult mice, including problem-solving tips. This protocol can be used to measure neuronal excitability and agonist-mediated Ca2+ signaling in cerebellar slices expressing a genetic Ca2+ reporter in all PNs, thus improving yield of data. For complete details on the use and execution of this profile, please refer to Dhanya and Hasan (2021). © 2021 The Author(s).

    Citation

    Sreeja Kumari Dhanya, Gaiti Hasan. Two photon imaging of calcium responses in murine Purkinje neurons. STAR protocols. 2022 Mar 18;3(1):101105

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    PMID: 35098161

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