Correlation Engine 2.0
Clear Search sequence regions

Sizes of these terms reflect their relevance to your search.

The melanocortin-3 receptor (MC3R) is an important regulator of energy homeostasis and inflammation in mammals. However, its function in teleost fish needs to be further explored. In this study, we characterized rainbow trout MC3R (rtMC3R), which encoded a putative protein of 331 amino acids. Phylogenetic and chromosomal synteny analyses showed that rtMC3R was closely related to bony fishes. Quantitative PCR (qPCR) revealed that the transcripts of rtMC3R were highly expressed in the brain and muscle. The cellular function of rtMC3R was further verified by the signal-pathway-specific luciferase reporter assays. Four agonists such as α-MSH, β-MSH, ACTH (1-24), and NDP-MSH can active rtMC3R, increasing the production of intracellular cAMP and upregulating MAPK/ERK signals. Moreover, we found that rtMC3R stimulated with α-MSH and NDP-MSH can significantly inhibit the NF-κB signaling pathway. This research will be helpful for further studies on the function of MC3R in rainbow trout, especially the role of energy metabolism and immune regulation. © 2021. The Author(s), under exclusive licence to Springer Nature B.V.


Hui-Xia Yu, Yang Li, Wei-Jia Song, Hui Wang, Hao-Lin Mo, Qiao Liu, Xin-Miao Zhang, Ze-Bin Jiang, Li-Xin Wang. Functional characterization of melanocortin-3 receptor in rainbow trout (Oncorhynchus mykiss). Fish physiology and biochemistry. 2022 Feb;48(1):241-252

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 35098384

View Full Text