Endothelial Cell Fibrin Gel Angiogenesis Bead Assay.

The fibrin gel angiogenesis bead assay provides a controlled in vitro setting for observing endothelial angiogenic sprouting in response to modified variables. Endothelial cells are coated onto microcarriers and embedded into a fibrin clot containing necessary growth factors. Following a 24-h incubation, endothelial sprouts are imaged using a light microscope. This method is useful for rapidly and affordably investigating the effects of genetic or chemical manipulation to endothelial function. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Eva M Clavane, Hannah A Taylor, Richard M Cubbon, Paul J Meakin. Endothelial Cell Fibrin Gel Angiogenesis Bead Assay. Methods in molecular biology (Clifton, N.J.). 2022;2441:321-327

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PMID: 35099748

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