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The intrinsically disordered membrane-bound selenoprotein s (selenos) takes part in the protein quality control pathway, vesicle trafficking, and NF-kB signaling. The reactive selenocysteine (Sec) at the penultimate position is responsible for its enzymatic activity. We report the preparation of the soluble segment as well as the full-length selenos using expressed protein ligation. This chapter discusses the practical considerations of expressed protein ligation using selenopeptides and describes our optimized procedure for the semi-synthesis of selenos. Copyright © 2022 Elsevier Inc. All rights reserved.


Rujin Cheng, Jun Liu, Vidyadhar Daithankar, Sharon Rozovsky. Applying selenocysteine-mediated expressed protein ligation to prepare the membrane enzyme selenoprotein S. Methods in enzymology. 2022;662:159-185

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PMID: 35101209

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