Hybridoma Screening by Antibody Capture: Detection of Intracellular Binding by Immunofluorescence.

Edward A Greenfield

Cold Spring Harbor protocols 2022 Feb 01

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Hybridoma screening by immunofluorescence stainings of whole cells can be adapted to screen for antibodies to internal proteins by permeabilizing the cells before applying the hybridoma supernatants. In this protocol, cells are attached to a solid support (glass slides), which makes them easy to manipulate and transfer between different reagent solutions. © 2022 Cold Spring Harbor Laboratory Press.

Citation

Edward A Greenfield. Hybridoma Screening by Antibody Capture: Detection of Intracellular Binding by Immunofluorescence. Cold Spring Harbor protocols. 2022 Feb 01;2022(2)