Correlation Engine 2.0
Clear Search sequence regions


  • atpases (2)
  • dccd (5)
  • escherichia coli (1)
  • ferment (1)
  • hydrogen ion (1)
  • hyfB (2)
  • hyfD (4)
  • HyfF (5)
  • ND2 (1)
  • ND4 (1)
  • ND5 (1)
  • potassium (6)
  • protein family (1)
  • proton (9)
  • proton pump (1)
  • subunit (4)
  • Sizes of these terms reflect their relevance to your search.

    Escherichia coli anaerobically ferment glucose and perform proton/potassium exchange at pH 7.5. The role of hyf (hydrogenase 4) subunits (HyfBDF) in sensing different concentrations of glucose (2 g L-1 or 8 g L-1) via regulating H+/K+ exchange was studied. HyfB, HyfD and HyfF part of a protein family of NADH-ubiquinone oxidoreductase ND2, ND4 and ND5 subunits is predicted to operate as proton pump. Specific growth rate was optimal in wild type and mutants grown on 2 g L-1 glucose reaching ~ 0.8 h-1. It was shown that in wild type cells proton but not potassium fluxes were stimulated ~ 1.7 fold reaching up to 1.95 mmol/min when cells were grown in the presence of 8 g L-1 glucose. Interestingly, cells grown on peptone only had similar proton/potassium fluxes as grown on 2 g L-1glucose. H+/K+ fluxes of the cells grown on 2 g L-1 but not 8 g L-1 glucose depend on externally added glucose concentration in the assays. DCCD-sensitive H+ fluxes were tripled and K+ fluxes doubled in wild type cells grown on 8 g L-1 glucose compared to 2 g L-1 when in the assays 2 g L-1glucose was added. Interestingly, in hyfF mutant when cells were grown on 2 g L-1glucose and in 2 g L-1 assays DCCD-sensitive fluxes were not determined compared to wild type while in hyfD mutant it was doubled reaching up to 0.657 mmol/min. In hyf mutants DCCD-sensitive K+ fluxes were stimulated in hyfD and hyfF mutants compared to wild type but depend on external glucose concentration. DCCD-sensitive H+/K+ ratio was equal to ~ 2 except hyfF mutant grown and assayed on 2 g L-1glucose while in 8 g L-1 conditions role of hyfB and hyfD is considered. Taken together it can be concluded that Hyd-4 subunits (HyfBDF) play key role in sensing glucose concentration for regulation of DCCD-sensitive H+/K+ fluxes for maintaining proton motive force generation. © 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

    Citation

    Liana Vanyan, Karen Trchounian. HyfF subunit of hydrogenase 4 is crucial for regulating FOF1 dependent proton/potassium fluxes during fermentation of various concentrations of glucose. Journal of bioenergetics and biomembranes. 2022 Apr;54(2):69-79

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 35106641

    View Full Text