Solid-Phase Synthesis and Application of a Clickable Version of Epoxomicin for Proteasome Activity Analysis.

Andres F Salazar-Chaparro, Saayak Halder, Marianne E Maresh, Darci J Trader

Chembiochem : a European journal of chemical biology 2022 Apr 05

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Degradation of proteins by the proteasome is an essential cellular process and one that many wish to study in a variety of disease types. There are commercially available probes that can monitor proteasome activity in cells, but they typically contain common fluorophores that limit their simultaneous use with other activity-based probes. In order to exchange the fluorophore or incorporate an enrichment tag, the proteasome probe likely has to be synthesized which can be cumbersome. Here, we describe a simple synthetic procedure that only requires one purification step to generate epoxomicin, a selective proteasome inhibitor, with a terminal alkyne. Through a copper-catalyzed cycloaddition, any moiety containing an azide can be incorporated into the probe. Many fluorophores are commercially available that contain an azide that can be "clicked", allowing this proteasome activity probe to be included into already established assays to monitor both proteasome activity and other cellular activities of interest. © 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH.

Citation

Andres F Salazar-Chaparro, Saayak Halder, Marianne E Maresh, Darci J Trader. Solid-Phase Synthesis and Application of a Clickable Version of Epoxomicin for Proteasome Activity Analysis. Chembiochem : a European journal of chemical biology. 2022 Apr 05;23(7):e202100710