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DNA vaccination is one of the most fascinating vaccine strategies currently in development. Two of the main advantages of DNA immunization rely on its simplicity and flexibility, being ideal to dissect both the immune mechanisms and the antigens involved in protection against a given pathogen. Here we describe several strategies used to enhance the immune responses induced and the protection afforded by experimental DNA vaccines tested in swine and provide very basic protocols describing the generation and in vivo application of a prototypic DNA vaccine. The future will say the last word regarding the definitive implementation of DNA vaccination in the field. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


Francesc Accensi, Laia Bosch-Camós, Paula L Monteagudo, Fernando Rodríguez. DNA Vaccines in Pigs: From Immunization to Antigen Identification. Methods in molecular biology (Clifton, N.J.). 2022;2465:109-124

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PMID: 35118618

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