Dissection, Fixation, and Immunostaining of the Drosophila Midgut.

Jia Chen, Daniel St Johnston

Methods in molecular biology (Clifton, N.J.) 2022

filter terms:

The Drosophila midgut is mainly composed of highly polarized epithelial cells called enterocytes that establish their apical-basal polarity in a fundamentally different way from other Drosophila epithelia. The roles of polarity factors in the midgut can be studied by generating clones of homozygous mutant cells in the background of wild-type tissue. In this chapter, we will introduce and discuss the procedures for producing positively marked mutant clones in the midgut and describe specific protocols for dissecting, fixing, and immunostaining this tissue. © 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Citation

Jia Chen, Daniel St Johnston. Dissection, Fixation, and Immunostaining of the Drosophila Midgut. Methods in molecular biology (Clifton, N.J.). 2022;2438:309-321