Bioresource-derived tannic acid-supported immuno-network in lateral flow immunoassay for sensitive clenbuterol monitoring.

Sijie Liu, Rui Shu, Chen Nie, Yuechun Li, Xing Luo, Yanwei Ji, Xuechi Yin, Jing Sun, Daohong Zhang, Jianlong Wang

Food chemistry 2022 Jul 15

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Although lateral flow immunoassay (LFIA) as a point-of-care (POC) diagnostic tool has been widely used because of numerous merits, it remains challenging to realize higher sensitivity, easier labeling, and fewer antibodies consumption. Herein, a bioresource-derived tannic acid (TA)-supported immuno-network based LFIA for clenbuterol (CLE) monitoring was proposed by employing poly TA nanospheres (PTAN) as the new tracer. Attributing to the effective protein-enrichment ability of TA, plenty of goat anti-mouse immunoglobulins (GAMI) were first absorbed on the surface of PTAN and then monoclonal antibodies (AbCLE) were added to form immuno-network. Compared with directly coupling antibodies, the utilization efficiency of AbCLE was significantly improved. Thus, the detection sensitivity (take the cut-off value as an example) was enhanced 5-fold and 10-fold at least compared with PTAN-AbCLE and colloidal gold-based LFIA, respectively. Additionally, the proposed method was successfully verified in beef and pork liver and provided an effective strategy for food safety monitoring. Copyright © 2022 Elsevier Ltd. All rights reserved.

Citation

Sijie Liu, Rui Shu, Chen Nie, Yuechun Li, Xing Luo, Yanwei Ji, Xuechi Yin, Jing Sun, Daohong Zhang, Jianlong Wang. Bioresource-derived tannic acid-supported immuno-network in lateral flow immunoassay for sensitive clenbuterol monitoring. Food chemistry. 2022 Jul 15;382:132390