The Effect of α-Tocopherol on the Reduction of Inflammatory Processes and the Negative Effect of Acrylamide.

Marta Kopańska, Marta Batoryna, Agnieszka Banaś-Ząbczyk, Joanna Błajda, Marcin W Lis

Molecules (Basel, Switzerland) 2022 Jan 31

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Our research aimed to show acrylamide's influence on inflammatory processes, the oxidative stress it causes in the cholinergic system, and the possibility of reducing inflammation via supplementation with α-tocopherol. For this purpose, an in ovo model was used where the embryos were exposed to acrylamide, α-tocopherol and a cocktail of these substances. After 48 h of exposure, we collected brain samples and performed biochemical assays to examine the effect of the chosen substances on oxidative stress (malondialdehyde-MDA and reduced glutathione-GSH) and acetylcholinesterase activity (AChE). The results showed that acrylamide decreased AChE activity in the examined brain samples by about 25% in comparison to the control group, and this effect was decreased by administering α-tocopherol. The concentration of malondialdehyde significantly increased in the group given acrylamide, while, in the group with α-tocopherol, the observed concentration was lower in comparison to the control group. Moreover, a decrease in glutathione concentration was observed after the administration of acrylamide; however, the protective effect of α-tocopherol was only slightly visible in this case. In conclusion, α-tocopherol minimizes the harmful effects of acrylamide on AchE, and it can minimize the concentration of MDA.

Citation

Marta Kopańska, Marta Batoryna, Agnieszka Banaś-Ząbczyk, Joanna Błajda, Marcin W Lis. The Effect of α-Tocopherol on the Reduction of Inflammatory Processes and the Negative Effect of Acrylamide. Molecules (Basel, Switzerland). 2022 Jan 31;27(3)