UV-Spectroscopic Detection of (Pyro-)Phosphate with the PUB Module.

Despite the prevalence of ortho- and pyrophosphate in biochemistry, operationally simple and versatile high-throughput methodologies for their quantification are lacking. We herein introduce PUB, a module for phosphate detection by continuous UV-spectroscopic monitoring of 5-bromouridine phosphorolysis. The PUB module uses cheaply available, bench-stable reagents and can be employed for continuous and discontinuous reaction monitoring in biochemical assays to detect (pyro-)phosphate concentrations spanning almost 4 orders of magnitude, as demonstrated with representative use cases.

Citation

Lea Eilert, Anett Schallmey, Felix Kaspar. UV-Spectroscopic Detection of (Pyro-)Phosphate with the PUB Module. Analytical chemistry. 2022 Mar 01;94(8):3432-3435

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PMID: 35166519

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