Crystal structure of a tandem B-box domain from Arabidopsis CONSTANS.

Pawan Dahal, Eunju Kwon, Deepak Pathak, Dong Young Kim

Biochemical and biophysical research communications 2022 Apr 09

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CONSTANS is a central protein in the regulation of photoperiodic flowering, which is expressed in response to day length and promotes the expression of Flowering Locus T. The tandem B-box domain in CONSTANS mediates interactions with various proteins to regulate the expression of Flowering Locus T. Although most plants, including Arabidopsis, have multiple B-box proteins, their B-box structures have not been elucidated. Here, we report the crystal structure of a tandem B-box domain from Arabidopsis CONSTANS. The crystal structure shows that each B-box adopts a canonical B-box fold and coordinates two zinc atoms. Furthermore, the crystal structure reveals that the B-box domain has a unique structure that distinguishes it from animal B-boxes at the monomer and dimer level. Copyright © 2022 Elsevier Inc. All rights reserved.

Citation

Pawan Dahal, Eunju Kwon, Deepak Pathak, Dong Young Kim. Crystal structure of a tandem B-box domain from Arabidopsis CONSTANS. Biochemical and biophysical research communications. 2022 Apr 09;599:38-42