In vitro and in vivo wound healing-promoting activities of phosvitin-derived peptide Pt5-1c.

Fei Wu, Yi Gong, Lili Song, Haoyi Li, Xiangmin Zhang, Hongyan Li, Shicui Zhang

European journal of pharmacology 2022 Apr 05

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The closure of skin wounds is indispensable for resistance against pathogens, and fibroblast plays a critical role in skin wound healing. Our previous study demonstrates that the phosvitin-derived small peptide Pt5-1c not only possesses broad-spectrum antimicrobial activity but also exhibits synergistic effect and antibiofilm activity with traditional antibiotics against bacteria, including multi-drug resistant (MDR) strains. Here we provided the first evidence that Pt5-1c promoted the wound closure of surrogate scratch "wounds" of fibroblasts in vitro, and speeded up the healing and re-epithelialization of murine dermal wounds in vivo. We also showed that Pt5-1c activated migration of fibroblasts via a combined action of inducing migratory phenotype and trans-activating epidermal growth factor receptor (EGFR). Moreover, Pt5-1c accelerated attachment and proliferation of fibroblasts in vitro. Interestingly, Pt5-1c was able to promote collagen contraction through activation/differentiation of fibroblasts into myofibroblasts. These data together suggest that Pt5-1c is a promising candidate with therapeutic potential to promote wound healing. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Fei Wu, Yi Gong, Lili Song, Haoyi Li, Xiangmin Zhang, Hongyan Li, Shicui Zhang. In vitro and in vivo wound healing-promoting activities of phosvitin-derived peptide Pt5-1c. European journal of pharmacology. 2022 Apr 05;920:174833