Engineered Halomonas spp. for production of l-Lysine and cadaverine.

Cuihuan Zhao, Taoran Zheng, Yinghao Feng, Xuan Wang, Lizhan Zhang, Qitiao Hu, Jinchun Chen, Fuqing Wu, Guo-Qiang Chen

Bioresource technology 2022 Apr

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Cadaverine, a derivative of l-lysine, has been used as a monomer for the synthesis of bio-based nylon-5,6. This study engineered Halomonas bluephagenesis TD1.0 by blocking the feedback inhibition, overexpressing the key l-lysine synthesis genes, strengthening the l-lysine export system and increasing the supply of oxaloacetate for production of l-lysine in the supernatant and PHB in the cells. Subsequently, cadaverine biosynthetic pathway was constructed in H. campaniensis LC-9 to improve the efficiency of de novo cadaverine biosynthesis which combines l-lysine producing H. bluephagenesis TDL8-68-259 and cadaverine producing H. campaniensis LC-9-ldcC-lysP. When H. campaniensis LC-9-ldcC-lysP was used as a whole cell catalysis for cadaverine production, the conversion efficiency of l-lysine to cadaverine reached 100% in the presence of 0.05% Triton X-100 for cell membrane permeability enhancement, resulting in 118 g L-1 cadaverine formed in the fermentor. Thus, Halomonas spp. have been successfully constructed for l-lysine and cadaverine production. Copyright © 2022 Elsevier Ltd. All rights reserved.

Citation

Cuihuan Zhao, Taoran Zheng, Yinghao Feng, Xuan Wang, Lizhan Zhang, Qitiao Hu, Jinchun Chen, Fuqing Wu, Guo-Qiang Chen. Engineered Halomonas spp. for production of l-Lysine and cadaverine. Bioresource technology. 2022 Apr;349:126865