Protein expression of P-glycoprotein in neocortex from patients with frontal lobe epilepsy.

Epilepsy research 2022 Mar

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Frontal lobe epilepsy (FLE) is the second most frequent type of epilepsy and the surgical outcome depends on the etiology. For instance, patients with posttraumatic FLE (PTE) have a worse surgical outcome compared to patients with FLE related to a tumoral lesion (TL). The present study focuses to determine if the FLE etiology is associated with the P-glycoprotein (P-gp) expression, a condition associated with drug resistance. P-gp expression and cellular localization were determined by Western Blot and immunohistochemical experiments in cortical brain samples obtained from patients with PTE (n = 5), TL (n = 5), and autopsies (n = 5). The neuronal count was estimated by Nissl and stereology procedure. Results showed that the autopsies tissue showed a neuronal count of 3514 ± 304.2 neurons per mm3. The P-gp expression ratio was 0.33 ± 0.02. Its expression was found in endothelial cells. Negligible P-gp expression was detected in neurons and astrocytes. Compared to the autopsies group, the TL group showed no changes in the neuronal count but, there was a decreased P-gp expression ratio (46%, p < 0.05). P-gp was located mainly in neurons, slight in astroglial, and endothelial cells. The PTE group showed a similar P-gp expression ratio compared to the autopsies group. P-gp was expressed in neurons, astrocytes, and endothelial cells in these samples. However, experiments revealed a high P-gp expression in a lower neuronal count (38%, p < 0.05 vs autopsy group). The present study reveals that patients with PTE present neuronal P-gp overexpression. This finding could underlie their worst surgical outcome. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Daniel Fonseca-Barriendos, Daniel Pérez-Pérez, Monserrat Fuentes-Mejía, Sandra Orozco-Suárez, Mario Alonso-Vanegas, Iris E Martínez-Juárez, Rosalinda Guevara-Guzmán, José Luis Castañeda-Cabral, Luisa Rocha. Protein expression of P-glycoprotein in neocortex from patients with frontal lobe epilepsy. Epilepsy research. 2022 Mar;181:106892