Direct effect of lipopolysaccharide and histamine on permeability of the rumen epithelium of steers ex vivo.

Shengtao Gao, Alateng Zhula, Wenhui Liu, Zhongyan Lu, Zanming Shen, Gregory B Penner, Lu Ma, Dengpan Bu

Journal of animal science 2022 Feb 01

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Disruption of the ruminal epithelium barrier occurs during subacute ruminal acidosis due to low pH, hyper-osmolality, and increased concentrations of lipopolysaccharide and histamine in ruminal fluid. However, the individual roles of lipopolysaccharide and histamine in the process of ruminal epithelium barriers disruption are not clear. The objective of the present investigation was to evaluate the direct effect of lipopolysaccharide and histamine on the barrier function of the ruminal epithelium. Compared with control (CON), histamine (HIS, 20 μM) increased the short-circuit current (Isc; 88.2%, P < 0.01), transepithelial conductance (Gt; 29.7%, P = 0.056), and the permeability of fluorescein 5(6)-isothiocyanate (FITC) (1.04-fold, P < 0.01) of ruminal epithelium. The apparent permeability of LPS was 1.81-fold higher than HIS (P < 0.01). The mRNA abundance of OCLN in ruminal epithelium was decreased by HIS (1.1-fold, P = 0.047). The results of the present study suggested that mucosal histamine plays a direct role in the disruption of ruminal epithelium barrier function, whereas lipopolysaccharide (at a pH of 7.4) has no effect on the permeability of rumen tissues ex vivo. © The Author(s) 2022. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Citation

Shengtao Gao, Alateng Zhula, Wenhui Liu, Zhongyan Lu, Zanming Shen, Gregory B Penner, Lu Ma, Dengpan Bu. Direct effect of lipopolysaccharide and histamine on permeability of the rumen epithelium of steers ex vivo. Journal of animal science. 2022 Feb 01;100(2)