Effects of vesicle-adipocyte interaction on regulation of insulin receptor recycling.

A Sandra, D J Fyler, S J Marshall

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme 1986 May

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The effect of interacting isolated rat adipocytes with small, unilammelar vesicles on insulin receptor internalization and processing was studied. Treatment of freshly isolated cells with vesicles containing phosphatidylcholine and phosphatidylserine followed by incubation in 35 mM Tris-containing buffer considerably reduced the chloroquine-induced increase in cell-associated 125I-insulin and significantly inhibited the time and insulin dependent loss of surface insulin receptors. The internal receptor pool, as measured by insulin binding to detergent solubilized adipocytes, was relatively smaller in vesicle-treated cells. Concomitant with a slower rate of receptor internalization, insulin-sensitive hexose uptake also demonstrated significantly slower kinetics of decreased response with time. These results support the conclusion that pretreatment of fat cells with phospholipid vesicles inhibits normal insulin receptor cycling.

Citation

A Sandra, D J Fyler, S J Marshall. Effects of vesicle-adipocyte interaction on regulation of insulin receptor recycling. Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme. 1986 May;18(5):285-9