The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking.

Anshul Milap Bhatt, Subba Rao Gangi Setty

Journal of visualized experiments : JoVE 2022 Feb 12

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Recycling endosomes (REs) are tubular-vesicular organelles generated from early/sorting endosomes in all cell types. These organelles play a key role in the biogenesis of melanosomes, a lysosome-related organelle produced by melanocytes. REs deliver the melanocyte-specific cargo to premature melanosomes during their formation. Blockage in the generation of REs, observed in several mutants of Hermansky-Pudlak syndrome, results in hypopigmentation of skin, hair, and eye. Therefore, studying the dynamics (refer to number and length) of REs is useful to understand the function of these organelles in normal and disease conditions. In this study, we aim to measure the RE dynamics using a resident SNARE STX13.

Citation

Anshul Milap Bhatt, Subba Rao Gangi Setty. The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking. Journal of visualized experiments : JoVE. 2022 Feb 12(180)