Effects of different carbon sources on metabolic profiles of carbohydrates in Streptococcus thermophilus during fermentation.

Xin Song, Chengjie Hou, Yong Yang, Liangzhong Ai, Yongjun Xia, Guangqiang Wang, Huaxi Yi, Zhiqiang Xiong

Journal of the science of food and agriculture 2022 Aug 30

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Streptococcus thermophilus is a major starter used in the dairy industry and it could improve the flavor of fermented products. It is necessary to improve biomass of S. thermophilus for its application and industrialization. The utilization of carbon sources directly affects the biomass of S. thermophilus. Therefore, the carbohydrate metabolism of S. thermophilus should be investigated. In the present study, metabolic parameters and gene expression of S. thermophilus S-3 with different carbon sources were investigated. The physicochemical results showed that S. thermophilus S-3 had high lactose utilization. Transcriptome analysis found that approximately 104 genes were annotated onto 15 carbohydrate metabolic pathways, of which 15 unigenes were involved in the phosphotransferase system and 75 were involved in the ATP-binding cassette transporter system. In addition, 171 differentially expressed genes related to carbohydrate metabolism were identified. Expression of the galactose metabolism genes lacSZ and galKTEM increased significantly from the lag phase to the mid-exponential growth phase as a result of the global regulator protein, catabolite control protein A (CcpA). The high expression of galK in the mid- to late- phases indicated that the metabolite galactose is re-transported for intracellular utilization. CcpA regulation may also induce high expressions of glycolytic pathway regulated-genes related to lactose utilization, including ldh, fba, eno, pfkA, bglA, pgi, pgm and pyk, producing optimal glycolytic flux and S. thermophilus S-3 growth. The present study provides new insights into the carbon metabolism regulation and provide theoretical support for high-density fermentation of S. thermophilus S-3. © 2022 Society of Chemical Industry. © 2022 Society of Chemical Industry.

Citation

Xin Song, Chengjie Hou, Yong Yang, Liangzhong Ai, Yongjun Xia, Guangqiang Wang, Huaxi Yi, Zhiqiang Xiong. Effects of different carbon sources on metabolic profiles of carbohydrates in Streptococcus thermophilus during fermentation. Journal of the science of food and agriculture. 2022 Aug 30;102(11):4820-4829