Cistanche promotes the adipogenesis of 3T3-L1 preadipocytes.

Ping Zhang, Le Su, Xiuyu Ji, Feifan Ma, Qiulin Yue, Chen Zhao, Song Zhang, Xin Sun, Kunlun Li, Lin Zhao

PloS one 2022

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Cistanche deserticola Ma (cistanche) is a traditional herb with a wide range of therapeutic properties. However, no evidence of cistanche's effect on adipogenesis has been found. The effect of cistanche that promotes the adipogenesis of 3T3-L1 preadipocytes was proved by using MTT spectrophotometry, Nile Red staining, Oil Red O staining and transcriptome sequencing technology. The mRNA level of key transcription factors for adipogenesis such as PPAR, AP2 and LPL were examined by RT-PCR. The results showed that the intracellular lipid content in cistanche treated cells were notably increased when compared with the non-treated cells. Between the differentiation and cistanche treated groups, the expression of adipogenesis related genes such as grow hormone releasing hormone (Ghrp), BCL2/adenovirus E1B interacting protein 3 (Bnip3) and Gastric inhibitory polypeptide receptor (Gipr) were significantly increased. Our findings also verified that cistanche promoted adipogenesis, which was accompanied by up-regulated level of Bnip3 and PPAR. This study could uncover new signaling pathways involved in adipogenesis regulation.

Citation

Ping Zhang, Le Su, Xiuyu Ji, Feifan Ma, Qiulin Yue, Chen Zhao, Song Zhang, Xin Sun, Kunlun Li, Lin Zhao. Cistanche promotes the adipogenesis of 3T3-L1 preadipocytes. PloS one. 2022;17(3):e0264772