Itol A May Affect the Growth and Development of Spodoptera frugiperda through Hijacking JHBP and Impeding JH Transport.

Lin Xu, Shan-Chi Yi, Jiu-Ying Li, Yao Tong, Cong Xie, Dong-Qiang Zeng, Wen-Wei Tang

Journal of agricultural and food chemistry 2022 Mar 16

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Isoryanodane and ryanodane diterpenes have a carbon skeleton correlation in structures, and their natural product-oxidized diterpenes show antifeedant and insecticidal activities against Hemiptera and Lepidoptera. While ryanodine mainly acts on the ryanodine receptor (RyR), isoryanodane does not. In this study, we demonstrated that itol A, an isoryanodane diterpenoid, could significantly downregulate the expression level of juvenile hormone-binding protein (JHBP), which plays a vital role in JH transport. RNAi bioassay indicated that silencing the Spodoptera frugipreda JHBP (SfJHBP) gene decreased itol A activity, which confirmed the developmental phenotypic observation. Parallel reaction monitoring (PRM) further confirmed that itol A affected JHBP's expression abundance. Although JHBP is not proven as the direct or only target of itol A, we confirmed that itol A's action effect depends largely on JHBP and that JHBP is a potential target of itol A. We present foundational evidence that itol A inhibits the growth and development of Spodoptera frugiperda mainly through hijacking JHBP.

Citation

Lin Xu, Shan-Chi Yi, Jiu-Ying Li, Yao Tong, Cong Xie, Dong-Qiang Zeng, Wen-Wei Tang. Itol A May Affect the Growth and Development of Spodoptera frugiperda through Hijacking JHBP and Impeding JH Transport. Journal of agricultural and food chemistry. 2022 Mar 16;70(10):3151-3161