Glutamate-cysteine ligase catalytic and its modifier function as novel immunotargets in gastric adenocarcinoma.

Dezhuan Da, Zhiang Pan, Lu Zeng, Yamei Dang, Chunyan Dang, Yunxia Huang, Dujuan Shi, Hongling Li

Asian journal of surgery 2023 Jan

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To determine the expression and function of glutamate-cysteine ligase catalytic (GCLC) and glutamate-cysteine ligase catalytic modifier (GCLM) in gastric adenocarcinoma. Bioinformatics was used to analyze the expression of GCLC and GCLM. We download and analyzed the expression of gastric adenocarcinoma patients from TCGA database. Moreover, the method of immunochemistry was used to verify the expression of GCLC and GCLM in gastric adenocarcinoma. At first, the expression of GCLC and GCLM in gastric adenocarcinoma tissues were both significantly higher compared with normal tissues analyzed via TCGA database. Then, gastric adenocarcinoma tissues were collected and performed with immunochemistry. The gastric adenocarcinoma with positive staining for GCLC and GCLM was 77% and 80%, respectively, which was significantly higher compared with adjacent normal tissues (9% and 11%, respectively). The disordered expression of GCLC and GCLM in gastric adenocarcinoma suggested that these factors may induce tumorigenesis and may be a novel target for diagnosis and treatment of gastric adenocarcinoma. Copyright © 2022 Asian Surgical Association and Taiwan Robotic Surgery Association. Published by Elsevier B.V. All rights reserved.

Citation

Dezhuan Da, Zhiang Pan, Lu Zeng, Yamei Dang, Chunyan Dang, Yunxia Huang, Dujuan Shi, Hongling Li. Glutamate-cysteine ligase catalytic and its modifier function as novel immunotargets in gastric adenocarcinoma. Asian journal of surgery. 2023 Jan;46(1):143-149