Ryuta Suzuki, Noriaki Kamio, Kozue Sugimoto, Shuichiro Maruoka, Yasuhiro Gon, Tadayoshi Kaneko, Yoshiyuki Yonehara, Kenichi Imai
In vivo (Athens, Greece) 2022 Mar-AprDespite evidence of an association between pulmonary diseases and periodontopathic bacteria, the molecular mechanisms remain unknown. Matrix metalloproteinase-9 (MMP9) plays important roles in pneumonia, chronic obstructive pulmonary disease, and asthma; therefore, we assessed the effects of Fusobacterium nucleatum on MMP9 expression in mouse lung and A549 human alveolar epithelial cells. Heat-killed F. nucleatum was administered to the trachea of mice or added to A549 cell cultures. MMP9 expression was determined using real-time PCR and western blotting. The involvement of mitogen-activated protein kinases (MAPKs) and nuclear factor-κB (NF-κB) in MMP9 expression was examined. F. nucleatum induced expression of MMP9 in mouse lung and bronchoalveolar lavage fluid. In A549 cells, F. nucleatum induced production of MMP9 protein and mRNA in a density-dependent manner; this was inhibited by inhibitors of extracellular-regulated kinase 1/2 and NF-κB, but not of p38 and Jun N-terminal protein kinase. F. nucleatum may contribute to the onset of pulmonary diseases via MMP9 expression through extracellular-regulated kinase 1/2 and NF-κB activation. Copyright © 2022, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
Ryuta Suzuki, Noriaki Kamio, Kozue Sugimoto, Shuichiro Maruoka, Yasuhiro Gon, Tadayoshi Kaneko, Yoshiyuki Yonehara, Kenichi Imai. Periodontopathic Bacterium Fusobacterium nucleatum Affects Matrix Metalloproteinase-9 Expression in Human Alveolar Epithelial Cells and Mouse Lung. In vivo (Athens, Greece). 2022 Mar-Apr;36(2):649-656
PMID: 35241518
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