Functional thermosensitive hydrogels based on chitin as RIN-m5F cell carrier for the treatment of diabetes.

Mingyu Zou, Jinhua Chi, Zhiwen Jiang, Wei Zhang, Huiwen Hu, Ruibao Ju, Chenqi Liu, Tianjiao Xu, Shuo Wang, Zhilong Feng, Wanshun Liu, Baoqin Han

International journal of biological macromolecules 2022 May 01

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Herein, the thermosensitive hydroxypropyl chitin (HPCT) hydrogel was prepared and the chemical structures, microstructures, rheological properties and degradation in vitro were investigated. The HPCT hydrogel possessed satisfactory biocompatibility in mouse fibroblast cells and Sprague Dawley rats. On the other hand, N-acetylglucosamine (NAG) and carboxymethyl chitosan (CMCS) provided favorable capacity for promoting cell proliferation, delaying cell apoptosis, and facilitating the insulin secretion of rat pancreatic beta cells (RIN-m5F) in three-dimensional culture. Most importantly, the effects of HPCT/NAG and HPCT/CMCS thermosensitive hydrogels as RIN-m5F cells carriers were evaluated via injection into different areas of diabetic rats. Our results demonstrated that HPCT/NAG and HPCT/CMCS hydrogels loaded RIN-m5F cells could keep cells survival, maintain insulin secretion and reduce blood glucose for one week. Overall, the functional thermosensitive hydrogels based on HPCT were effective cell carriers for RIN-m5F cells and might provide novel strategy for the treatment of diabetes via cell engineering. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Mingyu Zou, Jinhua Chi, Zhiwen Jiang, Wei Zhang, Huiwen Hu, Ruibao Ju, Chenqi Liu, Tianjiao Xu, Shuo Wang, Zhilong Feng, Wanshun Liu, Baoqin Han. Functional thermosensitive hydrogels based on chitin as RIN-m5F cell carrier for the treatment of diabetes. International journal of biological macromolecules. 2022 May 01;206:453-466