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Cell-free expression systems are becoming increasingly widely used due to their diverse applications in biotechnology. Despite this rapid expansion in adoption, many aspects of cell-free systems remain surprisingly poorly understood. Systems biology approaches make it possible to characterize cell-free systems deeply and broadly to better understand their underlying complexity. Here, we review recent systems biology studies that have provided insight into cell-free systems. We focus on characterization of the cell-free proteome, including its dependence on preparation protocol and host strain, as well as the cell-free metabolome and the relationship of endogenous metabolism to system performance. We conclude by highlighting promising future research directions. Copyright © 2022 Elsevier Ltd. All rights reserved.


Harini Sridharan, Fernanda Piorino, Mark P Styczynski. Systems biology-based analysis of cell-free systems. Current opinion in biotechnology. 2022 Jun;75:102703

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PMID: 35247659

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