Inhibiting tricin biosynthesis improves maize lignocellulose saccharification.

Lignin is a technological bottleneck to convert polysaccharides into fermentable sugars, and different strategies of genetic-based metabolic engineering have been applied to improve biomass saccharification. Using maize seedlings grown hydroponically for 24 h, we conducted a quick non-transgenic approach with five enzyme inhibitors of the lignin and tricin pathways. Two compounds [3,4-(methylenedioxy)cinnamic acid: MDCA and 2,4-pyridinedicarboxylic acid: PDCA] revealed interesting findings on root growth, lignin composition, and saccharification. By inhibiting hydroxycinnamoyl-CoA ligase, a key enzyme of phenylpropanoid pathway, MDCA decreased the lignin content and improved saccharification, but it decreased root growth. By inhibiting flavone synthase, a key enzyme of tricin biosynthesis, PDCA decreased total lignin content and improved saccharification without affecting root growth. PDCA was three-fold more effective than MDCA, suggesting that controlling lignin biosynthesis with enzymatic inhibitors may be an attractive strategy to improve biomass saccharification. Copyright © 2022 Elsevier Masson SAS. All rights reserved.

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Gabriela Galvão Machado Mendes, Thatiane Rodrigues Mota, Gabriela Ellen Barreto Bossoni, Rogério Marchiosi, Dyoni Matias de Oliveira, Rodrigo Polimeni Constantin, Wanderley Dantas Dos Santos, Osvaldo Ferrarese-Filho. Inhibiting tricin biosynthesis improves maize lignocellulose saccharification. Plant physiology and biochemistry : PPB. 2022 May 01;178:12-19

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PMID: 35247693

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