Inhibiting tricin biosynthesis improves maize lignocellulose saccharification.

Plant physiology and biochemistry : PPB 2022 May 01

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Lignin is a technological bottleneck to convert polysaccharides into fermentable sugars, and different strategies of genetic-based metabolic engineering have been applied to improve biomass saccharification. Using maize seedlings grown hydroponically for 24 h, we conducted a quick non-transgenic approach with five enzyme inhibitors of the lignin and tricin pathways. Two compounds [3,4-(methylenedioxy)cinnamic acid: MDCA and 2,4-pyridinedicarboxylic acid: PDCA] revealed interesting findings on root growth, lignin composition, and saccharification. By inhibiting hydroxycinnamoyl-CoA ligase, a key enzyme of phenylpropanoid pathway, MDCA decreased the lignin content and improved saccharification, but it decreased root growth. By inhibiting flavone synthase, a key enzyme of tricin biosynthesis, PDCA decreased total lignin content and improved saccharification without affecting root growth. PDCA was three-fold more effective than MDCA, suggesting that controlling lignin biosynthesis with enzymatic inhibitors may be an attractive strategy to improve biomass saccharification. Copyright © 2022 Elsevier Masson SAS. All rights reserved.

Citation

Gabriela Galvão Machado Mendes, Thatiane Rodrigues Mota, Gabriela Ellen Barreto Bossoni, Rogério Marchiosi, Dyoni Matias de Oliveira, Rodrigo Polimeni Constantin, Wanderley Dantas Dos Santos, Osvaldo Ferrarese-Filho. Inhibiting tricin biosynthesis improves maize lignocellulose saccharification. Plant physiology and biochemistry : PPB. 2022 May 01;178:12-19