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ID23-2 is a fixed-energy (14.2 keV) microfocus beamline at the European Synchrotron Radiation Facility (ESRF) dedicated to macromolecular crystallography. The optics and sample environment have recently been redesigned and rebuilt to take full advantage of the upgrade of the ESRF to the fourth generation Extremely Brilliant Source (ESRF-EBS). The upgraded beamline now makes use of two sets of compound refractive lenses and multilayer mirrors to obtain a highly intense (>1013 photons s-1) focused microbeam (minimum size 1.5 µm × 3 µm full width at half-maximum). The sample environment now includes a FLEX-HCD sample changer/storage system, as well as a state-of-the-art MD3Up high-precision multi-axis diffractometer. Automatic data reduction and analysis are also provided for more advanced protocols such as synchrotron serial crystallographic experiments. open access.


Max Nanao, Shibom Basu, Ulrich Zander, Thierry Giraud, John Surr, Matias Guijarro, Mario Lentini, Franck Felisaz, Jeremy Sinoir, Christian Morawe, Amparo Vivo, Antonia Beteva, Marcus Oscarsson, Hugo Caserotto, Fabien Dobias, David Flot, Didier Nurizzo, Jonathan Gigmes, Nicolas Foos, Ralf Siebrecht, Thomas Roth, Pascal Theveneau, Olof Svensson, Gergely Papp, Bernard Lavault, Florent Cipriani, Ray Barrett, Carole Clavel, Gordon Leonard. ID23-2: an automated and high-performance microfocus beamline for macromolecular crystallography at the ESRF. Journal of synchrotron radiation. 2022 Mar 01;29(Pt 2):581-590

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PMID: 35254323

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