Construction of full-length infectious cDNA clones of two Korean isolates of turnip mosaic virus breaking resistance in Brassica napus.

Zheng-Xing Song, Eun-Young Seo, Wen-Xing Hu, Jong-Hyeon Jeong, Jae Sun Moon, Kang-Hee Kim, Won-Seob Eom, In-Sook Cho, John Hammond, Hyoun-Sub Lim

Archives of virology 2022 Apr

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In this work, two new turnip mosaic virus (TuMV) strains (Canola-12 and Canola-14) overcoming resistance in canola (Brassica napus) were isolated from a B. napus sample that showed typical TuMV-like symptoms and was collected in the city of Gimcheon, South Korea, in 2020. The complete genome sequence was determined and an infectious clone was made for each isolate. Phylogenetic analysis indicated that the strains isolated from canola belonged to the World-B group. Both infectious clones, which used 35S and T7 promoters to drive expression, induced systemic symptoms in Nicotiana benthamiana and B. napus. To our knowledge, this is the first report of TuMV infecting B. napus in South Korea. © 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.

Citation

Zheng-Xing Song, Eun-Young Seo, Wen-Xing Hu, Jong-Hyeon Jeong, Jae Sun Moon, Kang-Hee Kim, Won-Seob Eom, In-Sook Cho, John Hammond, Hyoun-Sub Lim. Construction of full-length infectious cDNA clones of two Korean isolates of turnip mosaic virus breaking resistance in Brassica napus. Archives of virology. 2022 Apr;167(4):1157-1162