Interaction of p10/p27 with macrophage migration inhibitory factor promotes avian leukosis virus subgroup J infection.

Kunmei Yang, Jianhao Yang, Defang Zhou, Mingjun Zhu, Xusheng Du, Jing Zhou, Shenglong Liu, Ziqiang Cheng

Veterinary microbiology 2022 Apr

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Avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, induces myelocytoma and various other tumors in broilers and layers. Many recent studies have shown that ALV-J can hijack host molecules to facilitate infection. However, the molecular mechanisms of this process are not clear. Here, we aimed to elucidate the molecular mechanisms contributing to ALV-J infection. ALV-J hijacked MIF via p10 and p27 to facilitate ALV-J infection. ALV-J persistently activated MIF expression in DF-1 cells, and MIF significantly facilitated ALV-J internalization and replication, which demonstrated by MIF overexpression and knockdown experiments and treatment with the MIF antagonist ISO-1. Furthermore, we found that the two subunit proteins of Gag, p10 and p27, interacted with MIF in the cytoplasm, respectively. These results suggested that the p10 and p27 subunit in Gag protein recruited MIF to promote ALV-J infection, providing insights into the roles of the p10/p27 and the host factor MIF in ALV-J infection. The finding may facilitate the development of new strategies for controlling ALV-J or retrovirus infections. Copyright © 2022 Elsevier B.V. All rights reserved.

Citation

Kunmei Yang, Jianhao Yang, Defang Zhou, Mingjun Zhu, Xusheng Du, Jing Zhou, Shenglong Liu, Ziqiang Cheng. Interaction of p10/p27 with macrophage migration inhibitory factor promotes avian leukosis virus subgroup J infection. Veterinary microbiology. 2022 Apr;267:109389